Composite

Part:BBa_K1916119:Design

Designed by: Jonathan Chen   Group: iGEM16_UC_Davis   (2016-10-13)


Ara-Inducible CBCR8 Expression System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1239
    Illegal AgeI site found at 1329
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.


Source

Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.

References